Scientist - Protein Engineering

About Us

Monod Bio is a pioneer company in the design of de novo protein reagents for Life Sciences and Diagnostics. Our innovative design platform technology takes advantage of the most recent advances in deep learning computational protein engineering to create highly rapid and sensitive protein-based reagents and assays. Our visionary founding team and state-of-the-art technology and product development experience position Monod Bio at the forefront of molecular reporter, binder and functional protein innovation. We welcome exceptional individuals to join us in this journey.

About the Role

We are currently seeking a Scientist – Protein Engineering to help us lead the development of next-generation de novo protein reagents. This individual will drive protein design efforts from concept through implementation, working closely with a multidisciplinary team of computational designers, assay developers, and protein engineers. The ideal candidate will have deep expertise in protein engineering and biophysical characterization, along with a track record of success developing novel protein-based technologies.

Key Responsibilities

• Lead the design, screening, and characterization of de novoprotein reagents across a range of applications, from conceptualization through downstream implementation and validation
• Collaborate cross-functionally to align project objectives, timelines, and design needs with downstream applications
• Perform protein purification, including SEC, and carry out conjugation workflows
• Manage a catalog of protein reagents, defining and maintaining quality control and release criteria, as well as generating Certificates of Analysis
• Assess protein quality using techniques such as SDS-PAGE and SEC-MALS
• Characterize designed proteins using biophysical and biochemical methods, including binding kinetics (SPR, BLI), thermal stability (DSF/SLS), and secondary structure (CD)
• Maintain a comprehensive catalog of protein designs with corresponding characterization data, as well as a sample inventory. Oversee quality control and release criteria, generate Certificates of Analysis, and manage the shipment of protein materials to internal and external partners
• Identify and address challenges related to expression, aggregation, or stability, implementing optimization strategies as needed

• Contribute to data analysis and interpretation, clearly communicating findings and recommendations to internal teams
• Maintain detailed experimental records in Benchling and support documentation for internal and external reporting
• Stay current with advances in protein design and engineering, introducing new ideas and techniques to enhance team capabilities
• Other duties and projects as needed or required

Required Qualifications

• in molecular biology, biochemistry, bioengineering, or a related field, with 3+ years of relevant industry experience
• Proven track record in protein engineering, including structure-guided design, de novo design, and/or antibody/protein scaffold engineering
• Strong molecular biology skills, including PCR, cloning, Golden Gate or Gibson assembly
• Hands-on experience with protein expression and purification (primarily E. coli), including NiNTA, StrepTactin, and size exclusion chromatography
• Proficiency in biophysical and analytical methods: SPR/BLI, CD, SEC-MALS, DSF, SDS-PAGE, and Western blotting
• Strong understanding of the biophysics of protein structure/function relationships and confidence with structure modeling and visualization tools (e.g., PyMOL, Chimera, AlphaFold)
• Comfortable working in a fast-paced, interdisciplinary team; experience mentoring or managing others is a strong plus
• Strong communication skills and ability to present data and proposals to a range of audiences

Physical environment and key requirements

• Ability to perform work in both an office and laboratory environment. Will be in proximity to BSL2 labs. Familiarity with biological and chemical hazard handling. Training and equipment will be provided, as necessary
• Office and laboratory mobility, and the ability to stand for periods of time as needed is required
• Operations happen in moderately loud environments. PPE included by the company
• Mild odors from the manufacture of physical specimens and from laboratory work. The environment has been tested, and proper engineering protections are in place

We are proud to provide a generous compensation & benefits package, along with a healthy work culture

• 100% company-paid medical, dental, life/AD&D, and vision insurance premiums for employees scheduled to work 25+ hours per week. 50% paid premiums for eligible dependents
• 4 weeks PTO per year plus 11 paid holidays
• Parental leave benefit
• State-of-the-art lab and office on bus line and near coffee shops, restaurants, and South Lake Union neighborhood
• Casual environment, friendly colleagues, and of course, excellent snacks in the kitchen!

Every position requires certain physical capabilities with the major ones for this role noted above. Monod Bio seeks to provide reasonable accommodations that enable individuals with disabilities to perform essential duties when possible. Equal employment opportunity is a fundamental principle at Monod Bio. We are committed to nondiscrimination in all business operations. We are proud to enjoy a respectful and inclusive work environment where everyone has the opportunity to thrive.