Position Summary/ Department Summary:
The Ebrahimi-Fakhari Laboratory within the F.M. Kirby Neurobiology Center at Boston Children’s Hospital is seeking a highly motivated Research Assistant II to support translational neuroscience research focused on rare childhood-onset neurogenetic disorders, including hereditary spastic paraplegia.
The laboratory integrates patient-derived cellular systems, stem cell–based neuronal models, brain organoids, mouse models, and gene-based therapeutic development to advance disease-modifying strategies. The Research Assistant II will contribute to both experimental research (approximately 50%) and laboratory operations and regulatory coordination (approximately 50%).
This position is ideal for candidates seeking rigorous research training in a collaborative academic medical center and who are pursuing long-term careers in biomedical research, biotechnology, laboratory leadership, or graduate/medical training. A two-year commitment is strongly preferred.
Key Responsibilities:-
Perform and optimize molecular and cellular biology techniques (PCR, Western blotting, immunostaining, protein analysis)
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Maintain and support patient-derived fibroblast and neuronal cell culture systems
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Assist in design and execution of cellular trafficking and neurobiology assays
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Support mouse colony management, genotyping, and experimental procedures
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Analyze, organize, and present experimental data
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Coordinate day-to-day laboratory operations and workflow efficiency
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Maintain laboratory inventory systems, reagent databases, and procurement
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Monitor cryostorage systems and ensure accurate sample tracking
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Support preparation and renewal of animal and biosafety protocols
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Assist with regulatory compliance, safety procedures, and controlled substance documentation
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Serve as liaison with collaborators, vendors, and institutional offices
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Contribute to a collaborative and inclusive research environment
Minimum QualificationsEducation:-
Bachelor’s degree in neuroscience, biology, biochemistry, molecular biology, or a related scientific discipline required.
Experience:-
Minimum of 1-2 years of relevant laboratory research experience is required.
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Demonstrated proficiency in molecular and cellular biology techniques required.
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Experience with mammalian cell culture, patient-derived cell systems, or mouse models preferred.
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Experience working in a regulated research environment and supporting laboratory operations preferred.